The Impact of Exercise Serum on Selected Parameters of CD4+ T Cell Metabolism

CD4+ T cells are sensitive to peripheral changes of cytokine levels and metabolic substrates such as glucose lactate. This study aimed analyze whether factors released after exercise alter parameters human cell metabolism, specifically glycolysis oxidative phosphorylation. We used primary activated in the presence autologous serum, which was collected before (CO) a 30-min intervention (EX). In course activation, supernatants were analyzed for viability diameter, real-time oxygen consumption by using PreSens Technology, mRNA expression glycolytic enzymes complexes electron transport chain PCR, glucose, lactate supernatants, vitro differentiation flow cytometry. EX did not phenotype, viability, or on-blast formation. Similarly, no difference between CO found activation cellular consumption. contrast, higher 48 h conditions. serum expressed lower HK1 IFN-γ receptor 1. suggest that protocol sufficient destabilize immune metabolism cells. Therefore, more intense prolonged should be future studies.